The Telomeres and Telomerase Group, within the Molecular Oncology Programme, for the development of its research activity, needs to incorporate a postdoctoral fellow to identify the molecular mechanisms related with the fribotic development in kidney and liver. For the study of the role of the different cell populations in liver and kidney in the fibrosis pathology, we need to generate several “knock-outs” models of conditionals TRF1. The TRF1 deletion, specifically in cellular types in kidney and liver will allow us to characterize extensively the molecular processes that underlie to fibrosis. The mouse models generated will be studied at molecular, cellular and phenotypical level.
Se Requiere
- PhD in Pharmacy or Biomedical sciences (since 2012). Master on Biomedical Sciences.
- At least 8 years working with laboratory animals for experimentation.
- At least 10 years working in research laboratory.
- At least 10 scientific papers as first autor. We will also take into consideration all the publications in which the candidate has participated.
- Highly appreciated research stays in institutions outside candidate’s country.
- Technical skills:
• Phenotypic characterization of genetically modified embryos, neonatal and adult mice.
• Management and handling of laboratory animals, breeding and animal surgery.
• Tumor implantation and analysis (Orthotopic, xenograft, induced carcinogenesis tumor models).
• In vitro and in vivo models of angiogenesis (invasion, migration, tube formation, sponge implant, rat aortic assay, matrigel plug assay.
• Culture of several tumor and endothelial and embryonic cell lines.
• Extensive experience in epifluorescence, confocal and 2-photon microscopy, including live imaging Immunofluorescence, Confocal Microscopy.
• Flow cytometry. Experience in in vivo immunology, immune-regulation.
• Advanced skills in RNA expression analysis (qRT-PCR).
• Vast experience with different immunostaining, polyclonal antibody preparation, ELISA and in situ hybridization techniques.
• Extensive experience in molecular biology and various expression systems.
• Advanced skills in Biochemistry (westerns, immunoprecipitation, binding assays, internalization assays, protein production and purification).
• Extensive experience in chromatography (HPLC, GC, Gel filtration and TLC).
• Very good knowledge of general office, statistical (Graphpad) and imaging (Image J, Imaris) processing software.
- Fluent in English.
Se Ofrece
- The opportunity to be part of a Cancer Research Centre of excellence.
- Competitive salary.
- An energetic working environment.
- Contract linked to a project.
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