The Telomeres and Telomerase Group, within the Molecular Oncology Programme, needs a Postdoctoral Research Assistant for the design and execution of experiments addressed to studies on glioblastoma. The main objective of this job will be to study the role of the phosphorylation of telomeric proteins in the pathogenesis and treatment of glioblastoma.
Se Requiere
- PhD in Genetics and Molecular Biology (since 2016). Animal experimentation certificate (functions A, B, C).
- 7 years at least working in research laboratory, 3 years of laboratory experience after PhD.
- Fellowship obtained at the postdoctoral level. Attended at least one conference.
- 6 years of experience in the analysis of the post-translational modifications, included protein phosphorylation and ubiquitination.
- Experience in mammalian cell culture and cell manipulation.
- 6 years at least working in the telomeres field.
- Research experience with shelterin proteins.
- 3 publications in top-research journals.
- Technical skills:
• Biochemistry: Kinase Assay, Kinase assay coupled to mass spectrometry (MALDI-TOF), Immunoprecipitation, GST-Pulldown, Ubiquitin binding assay, Protein extraction, fractionation, expression, Western blotting, etc.
• Cell Biology: Mammalian and Drosophila cell cultures, cell transfection and RNA interference treatment, Retrovirus handling, etc.
• Genetics: Double mutant analysis, Recombination techniques.
• Molecular Biology: Band shift assay, Genomic DNA extraction, cDNA library production, Plasmidic DNA preparation (mini-, midi-, maxiprep), Chromatin Immunoprecipitation, Cloning techniques (restriction enzyme manipulation, ligation, TOPO-cloning, Gateway and Infusion Technologies), Mutagenesis techniques, PCR and Reverse Transcription PCR, RNA extraction, in vitro transcription.
• Basic Software and Databases technics.
- High level of English.
Se Ofrece
- The opportunity to be part of a Cancer Research Centre of excellence.
- Competitive salary.
- An energetic working environment.
- Contract linked to a project.
via CNIO. Empleo Más Información Aquí...
